New non-invasive test can help detect oral cancer, locate mouth & throat problems
The new method uses protein packages in the cells called exosomes.
By PTI |
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To analyse the vescicles, the researchers used a technique called Acoustofluidics -- an advanced technology that fuses the study of sound and fluids.
NEW YORK: Researchers have developed a new non-invasive method to analyse saliva for the presence of the human papilloma virus (HPV) which is associated with cancers of the mouth and throat.
The study, published in the Journal of Molecular Diagnostics, noted that the novel technique detected oropharyngeal cancers (OPCs) in whole saliva in 40 per cent of patients tested, and in 80 per cent of confirmed OPC patients.
The researchers, including those from Duke University in the US, said these cancers, which occur in the back of the mouth and upper throat, are often not diagnosed until they become advanced.
They said this is because their location makes it difficult to detect them during routine clinical exams.
"Considering these factors, the successful detection of HPV from salivary exosomes isolated by our acoustofluidic platform offers distinct advantages, including early detection, risk assessment, and screening," said study co-author Tony Jun Huang.
The scientists said the technique may also help physicians predict which patients will respond well to radiation therapy, or achieve longer progression-free survival.
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In the current study, they analysed saliva samples from 10 patients diagnosed with HPV-OPC using traditional methods.
The researchers, including those from Duke University in the US, said these cancers, which occur in the back of the mouth and upper throat, are often not diagnosed until they become advanced. The new method made use of the properties of tiny protein packages in the cells called exosomes.
They said these tiny microvesicles originating within cells, are secreted into body fluids.
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According to the researchers, the exosomes are believed to play a role in intercellular communication and their numbers are elevated in association with several types of cancers.
To analyse the vescicles, the researchers used a technique called Acoustofluidics -- an advanced technology that fuses the study of sound and fluids.
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The fluid samples were analyzed using a tiny acoustofluidic chip developed to isolate salivary exosomes by removing unwanted particles based on size.
This left behind exosome-rich concentrated samples that make it easier to detect tumor-specific biomarkers.
The researchers found that the new test identified the tumor biomarker HPV-16 DNA in 80 percent of the cases when coupled with another method called droplet digit PCR.
Since this method is independent of sample variability that arises due to changes in saliva viscosity, and collection methods used, the researchers said, it may prove ideal for use in clinical settings.
"With these features, the acoustofluidic technology has the potential to significantly exceed current industry standards, address unmet needs in the field, help expedite exosome-related biomedical research, and aid in the discovery of new exosomal biomarkers," Huang said.
Strawberry Toast, Pinenut Soup & Walnut Cake: Please Your Valentine With Mouth-Watering Delicacies
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Valentine's Day is just around the corner and there's so much that you can do to enjoy the perfect, romantic evening with your partner. A dinner date is a sure-shot win on Valentine's. However, this year, pledge to please your loved one with delicious treats prepared at home.
Top chefs share their secret recipes to make your celebration special.
Valentine's Day is just around the corner and there's so much that you can do to enjoy the perfect, romantic evening with your partner. A dinner date is a sure-shot win on Valentine's. However, this ..
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Executive Chef of the Jaypee Greens Golf & Spa Resort, Shivanand Kain, shared a cilantro rubbed roast chicken with strawberry and Californian grapes emulsion recipe to make Valentine's Day celebration more special.
Cilantro Rubbed Roast Chicken With Strawberry And Californian Grapes Emulsion
Ingredients Chicken Breast: 250 gm Grilled Vegetable: 80 gm Lyonnaise Potato: 80 gm Strawberry and Grapes Emulsion: 30 ml Fresh Coriander: 20 gm Chopped Parsley: 5 gm Olive Oil: 10 ml Chopped Garlic: 10 gm Salt: To taste Crushed Black Pepper: 2 gm
Method -Marinate the chicken breast with fresh coriander and garlic paste -Add seasoning, keep aside for 30 minutes -Grill the chicken breast, vegetables and lyonnaise potato -Arrange the grilled chicken breast on plate with vegetables and lyonnaise potatos -Pour strawberry and grapes emulsion -Garnish with parsley or herbs.
Executive Chef of the Jaypee Greens Golf & Spa Resort, Shivanand Kain, shared a cilantro rubbed roast chicken with strawberry and Californian grapes emulsion recipe to make Valentine's Day celebratio..
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Don't forget to bring an elegant dish on your Valentine's dinner table. We bring to you a Roasted Garlic & Pinenut Soup With Burnt Butter Essence recipe Straight from the Valentine's menu at San Gimignano Restaurant at The Imperial.
Roasted Garlic & Pinenut Soup With Burnt Butter Essence
Ingredients Garlic Cloves: 10 Whole Almonds: 5 Parsley: 15 gm Bayleaf: 1 Chicken Broth: 4 cups Nutmeg: A Pinch Egg Yolks: 1 Heavy Cream: Half a cup White Bread: 2 slices Butter: 1 tbsp Salt: To Taste Pepper: A Pinch White Wine: 30 ml
Method -Peel garlic cloves & blanch them -Add white wine, salt and butter and roast it for 20 minutes at 140 degree celcius -Take almonds and blanch them and remove their skin and also roast them -Make puree of roasted garlic and almonds -Heat a pan, add butter into it and then add puree of garlic and almond into pan -Add chicken broth and bouquet to it -Boil the soup -Whisk egg yolk and cream together -Pour the mixture into the soup -Stir to prevent the egg from curdling -Take the white bread to make croutons -Make burnt butter -Remove the bouquet garni from soup -Season with salt -Garnish with croutons and burnt butter.
Don't forget to bring an elegant dish on your Valentine's dinner table. We bring to you a Roasted Garlic & Pinenut Soup With Burnt Butter Essence recipe Straight from the Valentine's menu at San Gimi..
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Valentine's day celebration is incomplete without a mouthwatering dessert. Pullman & Novotel's Director of Culinary, Chef Ajay Anand, brings an innovative strawberry rasgulla dome, boondi ladoo srusle recipe to add the sweet element to your celebration.
Method -Heat strawberry puree and white chocolate together -Fold it with whipping cream and soaked gelatine leaves -Make a dome with a Rasgulla in the centre -Keep it to freeze -Glaze the dish with a red glaze- -Serve it along with a boondi strusel,raspberry curd and mint spring.
Valentine's day celebration is incomplete without a mouthwatering dessert. Pullman & Novotel's Director of Culinary, Chef Ajay Anand, brings an innovative strawberry rasgulla dome, boondi ladoo srusl..
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You simply can't consider a Valentine's Day celebration complete without a cake. F&B Director and Executive Chef of Radisson Blu Atria Bengaluru, Chef Kasiviswanathan, shares an easy-to-make recipe of an eggless chocolate walnut cake.
Method -Grease and dust an 8” baking tin and preheat oven to 180 degree celcius -Sieve together Maida, cocoa and baking soda -Beat butter and dahi until light and creamy -Fold in the sieved ingredients, alternating with milk and condensed milk -Fold in the chopped walnuts and pour into greased tin -Bake in the preheated oven for 25-30 minutes till tooth pick inserted in the centre comes out clean -Allow to cool in tin for 5 minutes and remove onto a wire rack.
For Chocolate Icing -Mix together butter, cocoa, condensed milk and beat well -Microwave for 20 seconds -Mix thoroughly -Spread over cake and leave to set -Slice and serve.
You simply can't consider a Valentine's Day celebration complete without a cake. F&B Director and Executive Chef of Radisson Blu Atria Bengaluru, Chef Kasiviswanathan, shares an easy-to-make recipe o..
