Scientists found rare microscopic Ice Age giant residues on ancient tools in the Carolinas, and it changed what they knew about early American hunters
Archaeologists in the Carolinas have found direct evidence of Ice Age hunters killing megafauna. Using advanced protein residue and microscopic wear analysis on ancient stone tools, scientists identified blood traces from mammoths and horses. This...

For archaeologists, it was rather difficult because they could estimate the age of those points and scrapers but lacked data as for the function of these Paleolithic artifacts. Nevertheless, nowadays, archaeologists can finally get something that is rare for them when working on eastern sites – direct evidence that megafauna were hunted by these ancient people using the given Paleolithic tools.
Evidence that was found by researchers
It was mentioned above that in 2023, a new paper was published in Scientific Reports regarding a new find. Using protein residue testing and microscopic analysis of the wear marks on the stones, scientists discovered traces of blood proteins of long-extinct animals such as proboscideans and ancient horses. Proboscideans refer to animals like mammoths and mastodons, among the biggest animal species living in North America at the time of the Ice Age.
From the microscopic analysis of wear patterns on the stone implements, scientists concluded the uses of those tools in throwing, butchering, skinning of animals and processing of meat. Both pieces of information were critical since residue analysis may not be always convincing; however, combination of wear marks and chemical evidence provides strong indications regarding the uses of tools. It proves that Paleoamerican societies inhabiting Carolinas engaged in hunting and using megafauna on their own.
Why the East was difficult to explore
Archaeological sites in the western part of North America have provided evidence of massive kills of extinct species, preserved skeletons, and hunting tools; however, eastern regions do not provide the same information. According to the researchers of the study, the soil in the area destroys bones of extinct animals, making it impossible to recognize them.
In effect, the issue of preservation, therefore, meant that researchers could not base any solid assumption concerning hunting in the region. The prevailing knowledge about the Clovis hunting in the Southeast was based mainly on indirect clues like the designs and positioning of tools. However, with the remaining evidence, there were changes to be considered because of the possibility to prove conclusions through direct biological residues on stones used for toolmaking. In eastern archaeology, where there are no animal bones left, the tools become behavioral markers.

Why microwear and residue helped
Microwear analysis methodologies had already been used elsewhere before. According to an article published in the Journal of Archaeological Science, residue analysis was conducted on ancient artifacts from Schöningen in Germany, which revealed meat-cutting, hide processing, and wood-working actions. The study in Carolinas was based on the same idea.
The significance of this study for Clovis disputes
Moreover, the study also contributes to the bigger discourse about the Clovis culture. Archaeologists have been discussing the purpose of Clovis points; whether they were made exclusively for the hunting of megafauna in the ice age or had other functions too. A modeling experiment that was published in Archaeological and Anthropological Sciences suggested that Clovis points were effective in megafauna hunting as well as in defense.
This study supports the above hypothesis from the Eastern United States. In the study, researchers identified proboscidean residues specifically tied to Clovis and early Paleoamerican projectile points. Other points yielded remain connected to other animal species, indicating varying hunting strategies and not a static hunting method. This indicates that the reality was much different compared to earlier conceptions.
Significance of the discovery for the Carolinas
The finding has also put the Carolinas on the map when it comes to archaeology. Recent studies on Paleoamerican fluted point traditions in the southeastern part of the U.S. have revealed that the region had a bigger part in early North American settlement patterns than originally thought. With this new discovery, it only adds credence to such beliefs.
The Carolina region is no longer seen merely as an area with poor preservation of artifacts but is now considered crucial to understand early American survival after their arrival on the continent. What the study brings to methodology. This was not an instantaneous discovery of an Ice Age graveyard. Rather, it was made possible by applying contemporary scientific methods to information that had been around within already discovered tools for some time.
The reason why this change is important is that it opens up the possibilities of finding additional information within other eastern tool collections. At the end of the day, however, the discovery did not just alter one particular interpretation of history. It demonstrated that while bones might vanish, human activity leaves its marks on stones.
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